Haji Khamis1*, Kituyi Lusweti2, Haji Mwevura3
1,2Department of Chemistry and Biochemistry, P.0.Box 1125-30100, University of Eldoret, Chepkoilel, Kenya
3The State University of Zanzibar (SUZA), P. O. Box 146, Zanzibar, Tanzania Corresponding author: Haji Khamis Email : email@example.com
Tomatoes contain lycopene and β-carotene which have important health benefits. Different varieties are available in Zanzibar Islands and used in daily meal. However, there is little information in Zanzibar about quantity of all trans-lycopene, cis-lycopene and β- carotene available in those tomato varieties. The objective of this study was to quantify all trans-lycopene, cis-lycopene and β- carotene from tomato varieties. Samples were collected and extracted using hexane/acetone/ethyl acetate (4:2:1 v/v/v). Extracts were filtered and lycopene layer separated, washed, dried, dissolved in hexane and fractionated by silica gel column. Fractions were collected, dried by blowing with a stream of nitrogen gas and measured their masses by electronic balance. Results showed that mass (in g/g) of all trans-lycopene varied from 316 to 827 and from 136 to 931, cis-lycopene from 60 to 528 and from 35 to 845, β- carotene from 41 to 405 and from 44.1 to 573, from Lycopersicon esculentum and Solanum quitoense respectively.Tomatoes contain valuable quantities of lycopene and β-carotene necessary for human nutrients. Best growing conditions which facilitate highest possible quantity of lycopeneand β-carotene should be investigated and used during tomato cultivation.
Keywords: lycopene, quantify, β-carotene, Lycopersicon esculentum, Solanum quitoense